 |
Recombinant |  |
Digest in 5 minutes |
 |
Enzymes that are Time-Saver qualified will digest unit assay substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions |  |
Digest in 15 minutes |
 |
Not completely digested in 15 minutes | ||
| NT | Not tested. |
| Enzyme | Sequence | Recombinant | Time-Saver Qualified | Unit Assay Substrate | Plasmid Substrate | PCR Substrate |
|---|---|---|---|---|---|---|
| AatII | GACGT/C |  |
 |
 |
 |
 |
| Acc65I | G/GTACC | |
|
|
|
|
| AccI | GT/MKAC | |
|
|
|
|
| AciI | CCGC(-3/-1) | |
|
|
|
|
| AclI | AA/CGTT | |
|
|
|
|
| AcuI | CTGAAG(16/14) | |
|
|
|
|
| AflII | C/TTAAG | |
|
|
|
|
| AgeI-HF® | A/CCGGT | |
|
|
|
|
| AhdI | GACNNN/NNGTC | |
|
|
|
|
| AluI | AG/CT | |
|
|
|
|
| AlwNI | CAGNNN/CTG | |
|
|
|
|
| ApaI | GGGCC/C | |
|
|
|
|
| ApaLI | G/TGCAC | |
|
|
|
|
| ApeKI | G/CWGC | |
|
|
|
|
| ApoI § | R/AATTY | |
|
|
|
|
| ApoI-HF | R/AATTY | |
|
|
|
|
| AscI | GG/CGCGCC | |
|
|
|
NT |
| AseI | AT/TAAT | |
|
|
|
NT |
| AvaI | C/YCGRG | |
|
|
|
|
| AvaII | G/GWCC | |
|
|
|
|
| AvrII | C/CTAGG | |
|
|
NT | NT |
| BaeGI | GKGCM/C | |
|
|
|
|
| BaeI | (10/15)ACNNNNGTAYC(12/7) | |
|
|
|
|
| BamHI § | G/GATCC | |
|
|
|
|
| BamHI-HF® | G/GATCC | |
|
|
|
|
| BbsI § | GAAGAC(2/6) | |
|
|
|
|
| BbsI-HF® | GAAGAC(2/6) | |
|
|
|
|
| BbvI | GCAGC(8/12) | |
|
|
|
|
| BccI | CCATC(4/5) | |
|
|
|
|
| BceAI | ACGGC(12/14) | |
|
|
|
|
| BciVI | GTATCC(6/5) | |
|
|
|
|
| BclI § | T/GATCA | |
|
|
|
|
| BclI-HF | T/GATCA | |
|
|
|
|
| BcoDI | GTCTC(1/5) | |
|
|
|
|
| BfuAI | ACCTGC(4/8) | |
|
|
|
|
| BglI | GCCNNNN/NGGC | |
|
|
|
|
| BglII | A/GATCT | |
|
|
|
|
| BlpI | GC/TNAGC | |
|
|
|
|
| BmgBI | CACGTC(-3/-3) | |
|
|
|
|
| BmrI | ACTGGG(5/4) | |
|
|
|
|
| BmtI-HF® | GCTAG/C | |
|
|
|
|
| BpuEI | CTTGAG(16/14) | |
|
|
|
|
| BsaAI | YAC/GTR | |
|
|
|
|
| BsaHI | GR/CGYC | |
|
|
|
|
| BsaI-HF®v2 | GGTCTC(1/5) | |
|
|
|
|
| BsaWI | W/CCGGW | |
|
|
|
|
| BsaXI | (9/12)ACNNNNNCTCC(10/7) | |
|
|
|
|
| BseRI | GAGGAG(10/8) | |
|
|
|
|
| BsgI | GTGCAG(16/14) | |
|
|
|
|
| BsiEI | CGRY/CG | |
|
|
|
|
| BsiHKAI | GWGCW/C | |
|
|
|
|
| BsiWI § | C/GTACG | |
|
|
|
|
| BsiWI-HF® | C/GTACG | |
|
|
|
|
| BslI | CCNNNNN/NNGG | |
|
|
|
|
| BsmAI | GTCTC(1/5) | |
|
|
|
|
| BsmBI | CGTCTC(1/5) | |
|
|
|
|
| BsmFI | GGGAC(10/14) | |
|
|
|
|
| BsmI | GAATGC(1/-1) | |
|
|
|
|
| BsoBI | C/YCGRG | |
|
|
|
|
| BspCNI | CTCAG(9/7) | |
|
|
|
|
| BspEI | T/CCGGA | |
|
|
|
|
| BspHI | T/CATGA | |
|
|
|
|
| Bsp1286I | GDGCH/C | |
|
|
|
|
| BspQI | GCTCTTC(1/4) | |
|
|
|
|
| BsrBI | CCGCTC(-3/-3) | |
|
|
|
|
| BsrDI | GCAATG(2/0) | |
|
|
|
|
| BsrFI-v2 | R/CCGGY | |
|
|
|
|
| BsrGI § | T/GTACA | |
|
|
|
|
| BsrGI-HF® | T/GTACA | |
|
|
|
|
| BsrI | ACTGG(1/-1) | |
|
|
|
|
| BssHII | G/CGCGC | |
|
|
|
|
| BssSI-v2 | CACGAG(-5/-1) | |
|
|
|
|
| BstBI | TT/CGAA | |
|
|
|
|
| BstEII § | G/GTNACC | |
|
|
|
|
| BstEII-HF® | G/GTNACC | |
|
|
|
|
| BstNI | CC/WGG | |
|
|
|
|
| BstUI | CG/CG | |
|
|
|
|
| BstXI | CCANNNNN/NTGG | |
|
|
|
|
| BstYI | R/GATCY | |
|
|
|
|
| BstZ17I-HF® | GTATAC | |
|
|
|
|
| Bsu36I | CC/TNAGG | |
|
|
|
|
| BtgI | C/CRYGG | |
|
|
|
|
| BtsCI | GGATG(2/0) | |
|
|
|
|
| BtsI-v2 | GCAGTG(2/0) | |
|
|
|
|
| Cac8I | GCN/NGC | |
|
|
|
|
| ClaI | AT/CGAT | |
|
|
|
|
| CspCI | (11/13)CAANNNNNGTGG(12/10) | |
|
|
|
|
| CviAII | C/ATG | |
|
|
|
|
| CviQI | G/TAC | |
|
|
|
|
| DdeI | C/TNAG | |
|
|
|
|
| DpnI | GA/TC | |
|
|
|
|
| DpnII | /GATC | |
|
|
|
|
| DraI | TTT/AAA | |
|
|
|
|
| DraIII-HF® | CACNNN/GTG | |
|
|
|
|
| DrdI | GACNNNN/NNGTC | |
|
|
|
|
| EagI § | C/GGCCG | |
|
|
|
|
| EagI-HF® | C/GGCCG | |
|
|
|
|
| EarI | CTCTTC(1/4) | |
|
|
|
|
| Eco53kI | GAG/CTC | |
|
|
|
|
| EcoNI | CCTNN/NNNAGG | |
|
|
|
|
| EcoO109I | RG/GNCCY | |
|
|
|
|
| EcoP15I | CAGCAG(25/27) | |
|
|
|
|
| EcoRI § | G/AATTC | |
|
|
|
|
| EcoRI-HF® | G/AATTC | |
|
|
|
|
| EcoRV § | GAT/ATC | |
|
|
|
|
| EcoRV-HF® | GAT/ATC | |
|
|
|
|
| Esp3I | CGTCTC(1/5) | |
|
|
|
|
| Fnu4HI | GC/NGC | |
|
|
|
|
| FokI | GGATG(9/13) | |
|
|
|
|
| FseI | GGCCGG/CC | |
|
|
|
|
| FspI | TGC/GCA | |
|
|
|
|
| HaeII | RGCGC/Y | |
|
|
|
|
| HaeIII | GG/CC | |
|
|
|
|
| HgaI | GACGC(5/10) | |
|
|
|
|
| HhaI | GCG/C | |
|
|
|
|
| HincII | GTY/RAC | |
|
|
|
|
| HindIII-HF® | A/AGCTT | |
|
|
|
|
| HinfI | G/ANTC | |
|
|
|
|
| HinP1I | G/CGC | |
|
|
|
|
| HpaII | C/CGG | |
|
|
|
|
| HphI | GGTGA(8/7) | |
|
|
|
|
| HpyAV | CCTTC(6/5) | |
|
|
|
NT |
| HpyCH4IV | A/CGT | |
|
|
|
|
| HpyCH4V | TG/CA | |
|
|
|
|
| Hpy166II | GTN/NAC | |
|
|
|
|
| KpnI § | GGTAC/C | |
|
|
|
|
| KpnI-HF® | GGTAC/C | |
|
|
|
|
| MboI | /GATC | |
|
|
|
|
| MboII | GAAGA(8/7) | |
|
|
|
|
| MfeI § | C/AATTG | |
|
|
|
|
| MfeI-HF® | C/AATTG | |
|
|
|
|
| MluCI | /AATT | |
|
|
|
|
| MluI § | A/CGCGT | |
|
|
|
|
| MluI-HF® | A/CGCGT | |
|
|
|
|
| MlyI | GAGTC(5/5) | |
|
|
|
|
| MmeI | TCCRAC(20/18) | |
|
|
|
|
| MnlI | CCTC(7/6) | |
|
|
|
|
| MseI | T/TAA | |
|
|
|
|
| MslI | CAYNN/NNRTG | |
|
|
|
|
| MspA1I | CMG/CKG | |
|
|
|
|
| MspI | C/CGG | |
|
|
|
|
| MwoI | GCNNNNN/NNGC | |
|
|
|
|
| NciI | CC/SGG | |
|
|
|
|
| NcoI § | C/CATGG | |
|
|
|
|
| NcoI-HF® | C/CATGG | |
|
|
|
|
| NdeI | CA/TATG | |
|
|
|
|
| NgoMIV | G/CCGGC | |
|
|
|
|
| NheI § | G/CTAGC | |
|
|
|
|
| NheI-HF® | G/CTAGC | |
|
|
|
|
| NlaIII | CATG/ | |
|
|
|
|
| NmeAIII | GCCGAG(21/19) | |
|
|
|
|
| NotI § | GC/GGCCGC | |
|
|
|
|
| NotI-HF® | GC/GGCCGC | |
|
|
|
|
| NruI § | TCG/CGA | |
|
|
|
|
| NruI-HF® | TCG/CGA | |
|
|
|
|
| NsiI § | ATGCA/T | |
|
|
|
|
| NsiI-HF® | ATGCA/T | |
|
|
|
|
| NspI | RCATG/Y | |
|
|
|
|
| PacI | TTAAT/TAA | |
|
|
|
|
| PaeR7I | C/TCGAG | |
|
|
|
|
| PflFI | GACN/NNGTC | |
|
|
|
|
| PflMI | CCANNNN/NTGG | |
|
|
|
|
| PmeI | GTTT/AAAC | |
|
|
|
NT |
| PmlI | CAC/GTG | |
|
|
|
|
| PpuMI | RG/GWCCY | |
|
|
|
|
| PshAI | GACNN/NNGTC | |
|
|
|
|
| PsiI-v2 | TTA/TAA | |
|
|
|
|
| PstI § | CTGCA/G | |
|
|
|
|
| PstI-HF® | CTGCA/G | |
|
|
|
|
| PvuI § | CGAT/CG | |
|
|
|
|
| PvuI-HF® | CGAT/CG | |
|
|
|
|
| PvuII § | CAG/CTG | |
|
|
|
|
| PvuII-HF® | CAG/CTG | |
|
|
|
|
| RsaI | GT/AC | |
|
|
|
|
| SacI § | GAGCT/C | |
|
|
|
|
| SacI-HF® | GAGCT/C | |
|
|
|
|
| SacII | CCGC/GG | |
|
|
|
|
| SalI § | G/TCGAC | |
|
|
|
|
| SalI-HF® | G/TCGAC | |
|
|
|
|
| SapI | GCTCTTC(1/4) | |
|
|
|
|
| SbfI § | CCTGCA/GG | |
|
|
|
|
| SbfI-HF® | CCTGCA/GG | |
|
|
|
|
| ScaI-HF® | AGT/ACT | |
|
|
|
|
| SfaNI | GCATC(5/9) | |
|
|
|
|
| SfiI | GGCCNNNN/NGGCC | |
|
|
|
|
| SfoI | GGC/GCC | |
|
|
|
|
| SmaI | CCC/GGG | |
|
|
|
|
| SpeI § | A/CTAGT | |
|
|
|
|
| SpeI-HF® | A/CTAGT | |
|
|
|
|
| SphI-HF® | GCATG/C | |
|
|
|
|
| SrfI | GCCC/GGGC | |
|
|
|
|
| SspI § | AAT/ATT | |
|
|
|
|
| SspI-HF® | AAT/ATT | |
|
|
|
|
| StuI | AGG/CCT | |
|
|
|
|
| StyD4I | /CCNGG | |
|
|
|
|
| StyI § | C/CWWGG | |
|
|
|
|
| StyI-HF® | C/CWWGG | |
|
|
|
|
| SwaI | ATTT/AAAT | |
|
|
|
|
| TaqI-v2 | T/CGA | |
|
|
|
|
| TfiI | G/AWTC | |
|
|
|
|
| TseI | G/CWGC | |
|
|
|
|
| TspMI | C/CCGGG | |
|
|
|
|
| TspRI | NNCASTGNN/ | |
|
|
|
|
| Tth111I | GACN/NNGTC | |
|
|
|
|
| XbaI | T/CTAGA | |
|
|
|
|
| XhoI | C/TCGAG | |
|
|
|
|
| XmaI | C/CCGGG | |
|
|
|
|
| XmnI | GAANN/NNTTC | |
|
|
|
|
§ An HF version of this enzyme is available.
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