The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of up to 20 μg of high quality plasmid DNA.
Learn more about Monarch DNA and RNA purification products
The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
View our videos on protocols, tips, and recycling Monarch.
|Culture Volume:||1-5 ml, not to exceed 15 OD units|
|Binding Capacity:||up to 20 μg|
|Plasmid Size:||up to 25 kb|
|Typical Recovery:||up to 20 μg. Yield depends on plasmid copy number, host
strain, culture volume, and growth conditions.
|Elution Volume:||≥ 30 μl|
|Purity:||A260/280 and A260/230 ≥ 1.8|
|Protocol Time:||10½ minutes of spin and incubation time|
|restriction digestion and other enzymatic manipulations,
transformation, transfection, DNA sequencing, PCR, labeling,
cell-free protein synthesis, etc.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Monarch® Plasmid Resuspension Buffer (B1)||25||1 X|
|Monarch® Plasmid Lysis Buffer (B2)||25||1 X|
|Monarch® Plasmid Neutralization Buffer (B3)||4||1 X|
|Monarch® Plasmid Wash Buffer 1||25||1 X|
|Monarch® Plasmid Wash Buffer 2||25||5 X|
|Monarch® DNA Elution Buffer||25||1 X|
|Monarch® Plasmid Miniprep Columns||25|
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