Nucleic Acid Purification
Biological workflows for detecting, analyzing, amplifying or manipulating DNA and RNA require extraction and purification from a biological sample and/or enzymatic reactions. DNA and RNA share similar biochemical structures, so purification methods are similar.
Monarch nucleic acid purification kits provide fast and reliable purification of high quality DNA and RNA from a variety of sources using best-in-class silica column technology. DNA and RNA purified with Monarch Kits is highly-pure and suitable for use in a wide variety of downstream applications including sequencing, cloning, and enzymatic manipulations. Available for plasmid minipreps, DNA gel extraction, PCR & DNA cleanup and total RNA extraction, Monarch kits are all designed with sustainability in mind; they use less plastic and are packaged in responsibly-sourced, recyclable material.
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- I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
- Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided in the Monarch DNA Gel Extraction Kit?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- Are Monarch spin columns compatible with Vacuum Manifolds?
- Can I use water to elute the DNA when using the Monarch Kits?
- Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)
- Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010)
- Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020)
- Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
- Monarch® RNA Cleanup Kit Protocol
- Quick Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010)
- Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
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Monarch Nucleic Acid Purification Brochure
It’s time to transform your DNA and RNA purification experience! Learn about the advantages of choosing Monarch Nucleic Acid Purification Kits from NEB.
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Dos and Don'ts of Plasmid Minipreps
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
- Guidelines for RNA Quantitation
- Guidelines for Working with RNA During RNA Cleanup
- Six Tips for a Perfect Gel Extraction
- Tips for the Monarch PCR & DNA Cleanup Kit
- Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids