Nucleic Acid Purification
Biological workflows for detecting, analyzing, amplifying or manipulating DNA and RNA often require extraction and purification from a biological sample and/or enzymatic reactions. Monarch nucleic acid purification kits provide fast and reliable purification of high quality DNA and RNA from a variety of sources using best-in-class silica column technology. DNA and RNA purified with Monarch Kits is highly-pure and suitable for use in a wide variety of downstream applications including sequencing, cloning, and enzymatic manipulations. Available for genomic DNA and total RNA extraction, DNA & RNA cleanup, plasmid minipreps, and gel extraction, Monarch kits are all designed with sustainability in mind; they use less plastic and are packaged in responsibly-sourced, recyclable material.
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- I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
- Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided in the Monarch DNA Gel Extraction Kit?
- Can I use a different elution buffer than the one supplied with the kit?
- What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
- Can I omit the low-speed binding centrifugation step to save time?
- Can I omit the RNase A digestion step from the protocol?
- How should I store purified gDNA samples?
- Does it matter which anticoagulant is used in blood samples?
- Is Monarch-purified gDNA suitable for long-range PCR?
- Can I use the Monarch® Genomic DNA Purification Kit to clean up gDNA that was extracted with phenol/chloroform?
- How can I assess gDNA integrity and purity?
- Can I use a different Proteinase K with the Monarch® Genomic DNA Purification Kit?
- Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
- When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
- Can I use Monarch® RNase A for applications/workflows other than gDNA purification?
- What is the minimal input amount that can be used with the Monarch® Genomic DNA Purification Kit?
- Can I use a different RNase A with the Monarch® Genomic DNA Purification Kit?
- Can I use the Monarch® Genomic DNA Purification Kit for gDNA cleanup?
- Can I get better recovery with the Monarch® Genomic DNA Purification Kit if I do a second elution with my eluent from the first elution?
- Can I use a different elution buffer than the one supplied with the kit?
- The enzymes in the kit were not stored at -20°C right away. Will they still work?
- What is the maximum binding capacity of the Monarch® Genomic DNA Purification Kit columns?
- What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
- How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
- What is the minimum elution volume that can be used with the Monarch® Genomic DNA Purification Kit columns?
- What is the DNA sequence of this product?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- Are Monarch spin columns compatible with Vacuum Manifolds?
- Can I use water to elute the DNA when using the Monarch Kits?
- Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)
- Genomic DNA Purification from Buccal Swabs (NEB #T3010)
- Genomic DNA Purification from Yeast (NEB #T3010)
- Genomic DNA Purification from Saliva (NEB #T3010)
- Genomic DNA Cleanup Protocol
- Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
- Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
- Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010)
- Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020)
- Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
- Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
- Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
- Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
- Quick Protocol for Extraction and Purification of Genomic DNA
- Monarch® RNA Cleanup Kit Protocol
- Quick Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- RNA Extraction from Cells
- RNA Extraction from Tissues
- Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
- RNA Extraction from Blood
- Quick Protocol for Total RNA Extraction (NEB #T2010)
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- Monarch Nucleic Acid Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
- Choosing RNA Input Amounts for NEB T2010
- Dos and Don'ts of Plasmid Minipreps
- Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit
- Guidelines for RNA Quantitation
- Guidelines for Working with RNA During RNA Cleanup
- Six Tips for a Perfect Gel Extraction
- Tips for the Monarch PCR & DNA Cleanup Kit
- Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids
Feature Articles
Brochures
Troubleshooting Guides
Usage Guidelines
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Monarch Plasmid Miniprep Kit protocol
Learn how to do a plasmid miniprep using the Monarch Plasmid Miniprep Kit.
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Monarch DNA Gel Extraction Kit protocol
Learn how to extract DNA from agarose gels using the Monarch DNA Gel Extraction Kit.
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Monarch® PCR & DNA Cleanup Kit Protocol
Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).
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Tips for using the Monarch Plasmid Miniprep Kit
Optimize your plasmid miniprep results with these quick tips for using the Monarch Plasmid Miniprep Kit.
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Tips for using the Monarch DNA Gel Extraction Kit
Optimize your DNA gel extractions with our quick tips for using the Monarch DNA Gel Extraction Kit.
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Tips for using the Monarch® PCR & DNA Cleanup Kit
Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit.
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Monarch Total RNA Miniprep Protocol
This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit.
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Tips for Successful RNA Purification using Monarch Total RNA Miniprep Kit
Optimize the results of your RNA extractions with these simple tips from our scientists.
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How to recycle your Monarch Kit components
Learn how you can easily recycle all of the components in your Monarch Kits.
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Tips for Handling and Storing Tissue Samples Prior to DNA Extraction
Successful genomic DNA extraction from tissues requires proper handling and storage upfront of purification. Follow our guidelines to ensure isolation of high quality, high molecular weight gDNA.
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Post-lysis Tips for Optimal Results using the Monarch Genomic DNA Purification Kit
After lysing your sample during your DNA extraction workflow, follow these tips to ensure that your purified gDNA is high-quality, intact, and free from contaminants.
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Tips for Efficient Lysis of Tissue Samples Using the Monarch Genomic DNA Purification Kit
Get helpful tips for effective lysis of tissue samples to optimize DNA extraction using the Monarch Genomic DNA Purification Kit
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Tips for Successful Genomic DNA Extraction from Blood Samples
Get helpful tips for DNA extraction from blood samples using the Monarch Genomic DNA Purification
