There are two steps to using this system: sub cloning and expression of the protein of interest as a CLIPf fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of the CLIPf-H2B fusion protein is described in this document. The labeling of fusion proteins with CLIP-tag substrates is described in the instructions supplied with CLIP-tag substrates.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
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