Home Markers & Ladders Products 1 kb DNA Ladder

1 kb DNA Ladder

This product is available in Quick-Load Purple format - enjoy the convenience of a ready-to-load format.

Size range: 500 bp to 10 kb
Value pricing
Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
Easy to identify reference bands
Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

Learn more about how this product is being used in the Product Citation Tool

Catalog #	Concentration	Size	List Price	Your Price
N3232L	500 µg/ml	1 ml	$213.00	Sign In Sign In
N3232S	500 µg/ml	0.2 ml	$54.00	Sign In Sign In

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Product Information

A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb). The 3.0 kb fragment has increased intensity to serve as a reference band. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

NEB also offers a Quick-Load version of this ladder with purple dye.

Recommended gel percentage range: 0.8-1.2%
Optimum separation on 1%

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Gel Loading Dye, Purple (6X), no SDS	25	6X

Product Categories:: DNA Markers & Ladders Products

Applications:: DNA Analysis

Properties & Usage

Bases

Fragment	Mass	bp
1	42	10,002
2	42	8,001
3	50	6,001
4	42	5,001
5	33	4,001
6	125	3,001
7	48	2,000
8	36	1,500
9	42	1,000
10a	21	517
10b	21	500

Effective Size Range

500bp to 10,002bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Related Products
Companion Products
Materials Sold Separately
- Gel Loading Dye, Purple (6X), no SDS
Product Notes
1. This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
2. We recommend loading 0.5 μg of 1 kb DNA Ladder diluted in sample buffer.
3. All fragments have a 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α-[³²P] dATP or α-[³²P] dTTP for the fill-in reaction.
4. 1 kb DNA Ladder is stable for at least 3 months at 4°C.
5. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂0.
6. 1X Gel Loading Dye, Purple (6X), no SDS:
  2.5% Ficoll®-400
  10mM EDTA
  3.3mM Tris-HCl (pH 8.0@25°C)
  0.02% Dye 1
  0.001% Dye 2
7. Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.
References
1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 10.51-10.67.

Protocols, Manuals & Usage
- Protocols
  End-labeling Protocol
  
  Suggested Loading Protocol for DNA Ladders & Markers
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N3232Datasheet-Lot1041202
  
  N3232Datasheet-Lot1051203
  
  N3232Datasheet-Lot1061205
  
  N3232Datasheet-Lot1071206
  
  N3232Datasheet-Lot1081208-1
  
  N3232Datasheet-Lot1091209
  
  N3232Datasheet-Lot1101211
  
  N3232Datasheet-Lot1131303
  
  N3232Datasheet-Lot1121302
  
  N3232Datasheet-Lot1141305
  
  N3232Datasheet-Lot1161308
  
  N3232Datasheet-Lot1171310
Tools & Resources
- Selection Charts
  DNA Markers & Ladders Selection Chart
FAQs & Troubleshooting
- FAQs
  Why are there extra bands visible on polyacrylamide gels?
  
  What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  
  Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  
  How can I quantify the amount of DNA in each band of a marker?
  
  Can I use GelRed with the DNA Ladders from NEB?
  
  Can I use Midori Green with the DNA Ladders from NEB?
  
  Can I use SYBR® with the DNA Ladders from NEB?
- Tech Tips
  
  To make it ready-to-load, dilute in TE buffer instead of water.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N3232S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N3232S_L_v1_1191401
  
  N3232S_L_v1_1201403
  
  N3232S_L_v1_1211404
  
  N3232S_L_v1_1221406
  
  N3232S_L_v1_1241409
  
  N3232S_L_v1_1231408
  
  N3232S_L_v1_1261501
  
  N3232S_L_v1_1271502
  
  N3232S_L_v1_1281504
  
  N3232S_L_v1_1251411
  
  N3232S_L_v1_1291506
  
  N3232S_L_v1_1301508
  
  N3232S_L_v1_1311509
  
  N3232S_L_v1_1321511
  
  N3232S_L_v1_1331512
  
  N3232S_L_v1_1341601
  
  N3232S_L_v1_1351603
  
  N3232S_L_v1_1361605
  
  N3232S_L_v1_1371607
  
  N3232S_L_v1_1381610
  
  N3232S_L_v1_1391612
  
  N3232S_L_v1_1401701
  
  N3232S_L_v1_1411704
  
  N3232S_L_v1_1421704
  
  N3232S_L_v1_1431707
  
  N3232S_L_v1_1441709
  
  N3232S_L_v1_1451711
  
  N3232S_L_v1_1461712
  
  N3232S_L_v1_1481803
  
  N3232S_L_v1_1471803
  
  N3232S_v1_10011837
  
  N3232L_v1_10012420
  
  N3232S_v1_10012422
  
  N3232L_v1_10019548
  
  N3232S_v1_10023272
  
  N3232L_v1_10027017
  
  N3232S_v1_10027019
  
  N3232L_v1_10033518
  
  N3232S_v1_10033520
  
  N3232L_v1_10039032
  
  N3232S_v1_10037289
  
  N3232S_v1_10040171
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  1 kb DNA Ladder
  
  Gel Loading Dye, Purple (6X), no SDS
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N3232Datasheet-Lot1041202
  
  N3232Datasheet-Lot1051203
  
  N3232Datasheet-Lot1061205
  
  N3232Datasheet-Lot1071206
  
  N3232Datasheet-Lot1081208-1
  
  N3232Datasheet-Lot1091209
  
  N3232Datasheet-Lot1101211
  
  N3232Datasheet-Lot1131303
  
  N3232Datasheet-Lot1121302
  
  N3232Datasheet-Lot1141305
  
  N3232Datasheet-Lot1161308
  
  N3232Datasheet-Lot1171310
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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