Home PCR, Polymerases & Amplification Technologies Products Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase is the industry standard for PCR

Ideal for routine PCR applications
Exceptional value in terms of cost per unit
Hot start version available

Learn more about how this product is being used in the Product Citation Tool

Catalog #	Concentration	Size	List Price	Your Price
M0273S	5,000 units/ml	400 units	$62.00	Sign In Sign In
M0273L	5,000 units/ml	2,000 units	$248.00	Sign In Sign In
M0273X	5,000 units/ml	4,000 units	$450.00	Sign In Sign In
M0273E	5,000 units/ml	20,000 units	$1,200.00	Sign In Sign In

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Product Information

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

Highlights

Isolated from a recombinant source
Supplied with 10X Reaction Buffer
Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Standard Taq Reaction Buffer Pack	-20	10X

Product Categories:: Taq DNA Polymerase Products

Applications:: Colony PCR,; A-tailing,; Polymerases for DNA Manipulation; Multiplex PCR; Specialty PCR; Routine PCR; PCR

Advantages and Features
Features

Application Features
- PCR
- Primer Extension
- DHPLC
- Microarray Analysis
- Colony PCR
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Standard Taq Reaction Buffer Pack

1X Standard Taq Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl₂
(pH 8.3 @ 25°C)

Storage Temperature
-20°C
Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Molecular Weight
Theoretical: 94000 daltons
5' - 3' Exonuclease
Yes
3' - 5' Exonuclease
No
Strand Displacement
+
Resulting Ends
- Single-base 3´ Overhangs
Unit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA
Error Rate
~ 285x10^-6bases
Related Products
Companion Products
Materials Sold Separately
- Standard Taq Reaction Buffer Pack
Product Notes
1. 5'→3' flap endonuclease degrades displaced strand.
References
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). NucleicAcids Res.. 18, 7465.

Protocols, Manuals & Usage
- Protocols
  PCR Protocol for Taq DNA Polymerase
  
  A-Tailing with Taq Polymerase
- Usage Guidelines
  Activity of Restriction Enzymes in PCR Buffers
  
  Guidelines for PCR Optimization with Taq DNA Polymerase
  
  Guidelines for PCR Optimization with Thermophilic DNA Polymerases
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0273Datasheet-Lot0141203
  
  M0273Datasheet-Lot0141209
  
  M0273Datasheet-Lot0141303
  
  M0273Datasheet-Lot0141309
  
  M0273Datasheet-Lot0141403
  
  M0273Datasheet-Lot0141408
  
  M0273Datasheet-Lot0141503
Tools & Resources
- Selection Charts
  DNA Polymerase Selection Chart
  
  PCR Selection Chart
  
  Taq Buffer Selection Chart
  
  Thermophilic DNA Polymerases
- Web Tools
  PCR Selector
  
  Tm Calculator
FAQs & Troubleshooting
- FAQs
  Can Taq DNA Polymerase be used in other buffers?
  
  How should I set up an amplification reaction using Taq DNA Polymerase?
  
  What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
  
  Why is the product a smear when visualized on an agarose gel?
  
  Why is there no product when visualized on an agarose gel?
  
  The product sequence doesn't completely match the expected sequence. How can this result be improved?
  
  When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  
  Does the presence of Ca2+ inhibit PCR?
  
  Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
  
  Can Taq DNA Polymerase be used for nick translation?
- Troubleshooting
  PCR Troubleshooting Guide
  
  Taq PCR Kit Troubleshooting Guide
- Tech Tips
  
  Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0273S_L_X_E_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0273S_L_X_E_v1_0141510
  
  M0273S_L_X_E_v1_0141512
  
  M0273S_L_X_E_v1_0141509
  
  M0273S_L_X_E_v1_0141606
  
  M0273S_L_X_E_v1_0141612
  
  M0273S_L_X_E_v1_0141705
  
  M0273S_L_X_E_v1_0141712
  
  M0273L_v1_10009935
  
  M0273S_v1_10012587
  
  M0273X_v1_10010822
  
  M0273E_v1_10017442
  
  M0273X_v1_10017005
  
  M0273E_v1_10017004
  
  M0273E_v1_10019200
  
  M0273X_v1_10019201
  
  M0273S_v1_10017271
  
  M0273L_v1_10017276
  
  M0273E_v1_10025772
  
  M0273S_v1_10023075
  
  M0273X_v1_10025773
  
  M0273E_v1_10032441
  
  M0273L_v1_10029413
  
  M0273E_v1_10033883
  
  M0273X_v1_10033882
  
  M0273X_v1_10034110
  
  M0273S_v1_10033881
  
  M0273E_v1_10036742
  
  M0273L_v1_10036016
  
  M0273S_v1_10036018
  
  M0273X_v1_10036029
  
  M0273E_v1_10041620
  
  M0273S_v1_10042935
  
  M0273E_v1_10043710
  
  M0273L_v1_10041493
  
  M0273E_v1_10047013
  
  M0273S_v1_10047008
  
  M0273X_v1_10042936
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Taq DNA Polymerase with Standard Taq Buffer
  
  Standard Taq Reaction Buffer Pack
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0273Datasheet-Lot0141203
  
  M0273Datasheet-Lot0141209
  
  M0273Datasheet-Lot0141303
  
  M0273Datasheet-Lot0141309
  
  M0273Datasheet-Lot0141403
  
  M0273Datasheet-Lot0141408
  
  M0273Datasheet-Lot0141503
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Trademarks
  
  NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  
  IGEPAL^®is a registered trademark of Rhodia Operations.
  TWEEN^®is a registered trademark of Uniqema Americas, LLC.

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