Deep Vent^® DNA Polymerase

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X ThermoPol^® Reaction Buffer Pack

1X ThermoPol^® Reaction Buffer Pack
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 89000 daltons

5' - 3' Exonuclease

No

3' - 5' Exonuclease

Yes

Strand Displacement

++

Resulting Ends

• Blunt Ends

Unit Assay Conditions

1X ThermoPol Reaction Buffer, 200 µM each dNTP including [³H]-dTTP and 15 nM primed M13 DNA.