Home PCR, Polymerases & Amplification Technologies Products Deep Vent^® DNA Polymerase

Deep Vent^® DNA Polymerase

Deep Vent^® offers moderate fidelity and robust performance
Extremely high thermostability: half-life of 23 hours at 95°C
5X higher fidelity than Taq
Difficult templates: ideal for GC-rich or looped sequence
Extremely High Thermostability: half-life of 23 hours at 95°C

Ordering Information

Catalog #

Concentration

2,000 units/ml

Size

200 units

List Price

$101.00

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Concentration

2,000 units/ml

Size

1,000 units

List Price

$406.00

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Quantity

Did you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.

Product Information

Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3´→ 5´ proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100°C.

Amplification of Jurkat genomic DNA with Deep Vent DNA Polymerase.

Product Source

An E. coli strain that carries the Deep Vent DNA Polymerase gene from Pyrococcus species GB-D. The native organism was isolated from a submarine thermal vent at 2,010 meters (1) and is able to grow at temperatures as high as 104°C.

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Magnesium Sulfate (MgSO₄) Solution	-20	100 mM
ThermoPol^®Reaction Buffer Pack	-20	10 X

Product Categories:: Other Pcr Polymerases Products

Applications:: Routine PCR,; PCR

Advantages and Features
Features
- Ideal for GC-rich or looped sequences
Application Features
- Primer extension
- PCR
Properties and Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Reaction Conditions

1X ThermoPol^® Reaction Buffer Pack

1X ThermoPol^® Reaction Buffer Pack
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Temperature
-20°C
Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation
No
Molecular Weight
Theoretical: 89000 daltons
5' - 3' Exonuclease
No
3' - 5' Exonuclease
Yes
Strand Displacement
++
Resulting Ends
- Blunt Ends
Unit Assay Conditions
1X ThermoPol Reaction Buffer, 200 µM each dNTP including [³H]-dTTP and 15 nM primed M13 DNA.
Related Products
Companion Products
Materials Sold Separately
- Magnesium Sulfate (MgSO₄) Solution
- ThermoPol® Reaction Buffer Pack
Notes
1. BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as NEB #B9001. Acetylated BSA should not be used for primer extension reactions.
2. The calculated half-life of Deep Vent DNA Polymerase at 95°C is 23 hours.
3. Diluent D is also available (NEB# B8004). This diluent is recommended for making dilutions of Deep Vent Polymerase.
4. Additional ThermoPol Reaction Buffer packs for this product are also available (NEB# B9004). Each buffer pack contains 4 vials of 10X buffer (1.5 mls each) and 1 vial of 100 mM MgSO₄.
References
1. Jannasch, H.W. et al. (1992). Appl. Environ. Microbiol.. 58, 3472-3481.

FAQs & Tech Tips
- FAQs
  Can Deep Vent DNA Polymerase be used in other buffers?
  
  I can't get Deep Vent DNA Polymerase to work yet Taq DNA Polymerase works fine.
  
  Are the DNA fragments produced by Deep Vent DNA Polymerase blunt-ended or do they have the single-base 3' overhang that Taq DNA Polymerase yields?
  
  Can I use Deep Vent DNA Polymerase to polish the ends of DNA fragments?
  
  I want to clone primer extension products made with Vent DNA Polymerase or Deep Vent DNA Polymerase. Are some protocols better than others?
  
  Can Vent® DNA Polymerase(NEB# M0254) or Deep Vent® DNA Polymerase (NEB# M0258) be used to create blunt ends on primer extension products made with Taq DNA Polymerase (NEB# M0267)?
- Tech Tips
  
  The amount of enzyme in the reaction is critical. Try a final concentration of 0.01 U/µl Deep Vent DNA Polymerase in the reaction.
Protocols & Manuals
- Protocols
  Guidelines for PCR Optimization for Deep Vent® DNA Polymerase
  
  Protocol for a Routine Deep Vent® PCR
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0258Datasheet-Lot0181206
  
  M0258Datasheet-Lot0181212
  
  M0258Datasheet-Lot0181406
  
  M0258Datasheet-Lot0181412
Other Tools & Resources
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0258S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0258S_L_v1_0181512
  
  M0258S_L_v1_0181606
  
  M0258S_L_v1_0181612
  
  M0258S_L_v1_0181702
  
  M0258S_L_v1_0181706
  
  M0258S_L_v1_0181712
  
  M0258L_v2_10015886
  
  M0258S_v2_10017725
  
  M0258L_v2_10028994
  
  M0258L_v2_10034648
  
  M0258S_v2_10034093
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Deep Vent^® DNA Polymerase
  
  Magnesium Sulfate (MgSO₄) Solution
  
  ThermoPol^®Reaction Buffer Pack
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0258Datasheet-Lot0181206
  
  M0258Datasheet-Lot0181212
  
  M0258Datasheet-Lot0181406
  
  M0258Datasheet-Lot0181412
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Licenses
  Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
  Trademarks
  NEW ENGLAND BIOLABS®, VENT® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  DEEP VENT™ is a trademark of New England Biolabs, Inc.
  
  TRITON® is a registered trademark of Union Carbide Corporation

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Applications

Product Categories

You’ll be Thrilled to Pieces

Tools

Resources

Deep Vent^® DNA Polymerase

Ordering Information

Product Source

Reagents Supplied

Features

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Companion Products

Materials Sold Separately

Licenses

Trademarks

Deep Vent® DNA Polymerase

Ordering Information

Product Source

Reagents Supplied

Features

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Companion Products

Materials Sold Separately

Licenses

Trademarks

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Deep Vent^® DNA Polymerase