Deep Vent® DNA Polymerase

Deep Vent^® DNA Polymerase

Deep Vent^® offers moderate fidelity and robust performance
Extremely high thermostability: half-life of 23 hours at 95°C
5X higher fidelity than Taq
Difficult templates: ideal for GC-rich or looped sequence
Extremely High Thermostability: half-life of 23 hours at 95°C

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Catalog #	Concentration	Size	List Price	Your Price
M0258S	2,000 units/ml	200 units	$103.00	Sign In Sign In
M0258L	2,000 units/ml	1,000 units	$414.00	Sign In Sign In

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Catalog #

Concentration

Size

List Price

Your Price

Quantity

M0258S

2,000 units/ml

200 units

$103.00

M0258L

2,000 units/ml

1,000 units

$414.00

Product Information

Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3´→ 5´ proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100°C.

Amplification of Jurkat genomic DNA with Deep Vent DNA Polymerase.

Product Source

An E. coli strain that carries the Deep Vent DNA Polymerase gene from Pyrococcus species GB-D. The native organism was isolated from a submarine thermal vent at 2,010 meters (1) and is able to grow at temperatures as high as 104°C.

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Magnesium Sulfate (MgSO₄) Solution	-20	100 mM
ThermoPol^® Reaction Buffer Pack	-20	10 X

Product Categories:: Other PCR Polymerases

Applications:: Routine PCR,; PCR

Advantages and Features
Features
- Ideal for GC-rich or looped sequences
Application Features
- Primer extension
- PCR
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Reaction Conditions

1X ThermoPol^® Reaction Buffer Pack

1X ThermoPol^® Reaction Buffer Pack
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Temperature
-20°C
Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation
No
Molecular Weight
Theoretical: 89000 daltons
5' - 3' Exonuclease
No
3' - 5' Exonuclease
Yes
Strand Displacement
++
Resulting Ends
- Blunt Ends
Unit Assay Conditions
1X ThermoPol Reaction Buffer, 200 µM each dNTP including [³H]-dTTP and 15 nM primed M13 DNA.
Related Products
Companion Products
- Deoxynucleotide (dNTP) Solution Set
- Deoxynucleotide (dNTP) Solution Mix
Materials Sold Separately
- Magnesium Sulfate (MgSO₄) Solution
- ThermoPol® Reaction Buffer Pack
Product Notes
1. BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as NEB #B9001. Acetylated BSA should not be used for primer extension reactions.
2. The calculated half-life of Deep Vent DNA Polymerase at 95°C is 23 hours.
3. Diluent D is also available (NEB# B8004). This diluent is recommended for making dilutions of Deep Vent Polymerase.
4. Additional ThermoPol Reaction Buffer packs for this product are also available (NEB# B9004). Each buffer pack contains 4 vials of 10X buffer (1.5 mls each) and 1 vial of 100 mM MgSO₄.
References
1. Jannasch, H.W. et al. (1992). Appl. Environ. Microbiol.. 58, 3472-3481.

Protocols, Manuals & Usage
- Protocols
  Guidelines for PCR Optimization for Deep Vent® DNA Polymerase
  
  Protocol for a Routine Deep Vent® PCR
- Usage Guidelines
  Guidelines for PCR Optimization with Thermophilic DNA Polymerases
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0258Datasheet-Lot0181206
  
  M0258Datasheet-Lot0181212
  
  M0258Datasheet-Lot0181406
  
  M0258Datasheet-Lot0181412
Tools & Resources
- Selection Charts
  DNA Polymerase Selection Chart
  
  Thermophilic DNA Polymerases
FAQs & Troubleshooting
- FAQs
  Can Deep Vent DNA Polymerase be used in other buffers?
  
  I can't get Deep Vent DNA Polymerase to work yet Taq DNA Polymerase works fine.
  
  Are the DNA fragments produced by Deep Vent DNA Polymerase blunt-ended or do they have the single-base 3' overhang that Taq DNA Polymerase yields?
  
  Can I use Deep Vent DNA Polymerase to polish the ends of DNA fragments?
  
  I want to clone primer extension products made with Vent DNA Polymerase or Deep Vent DNA Polymerase. Are some protocols better than others?
  
  Can Vent® DNA Polymerase(NEB# M0254) or Deep Vent® DNA Polymerase (NEB# M0258) be used to create blunt ends on primer extension products made with Taq DNA Polymerase (NEB# M0267)?
- Troubleshooting
  PCR Troubleshooting Guide
- Tech Tips
  
  The amount of enzyme in the reaction is critical. Try a final concentration of 0.01 U/µl Deep Vent DNA Polymerase in the reaction.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0258S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0258S_L_v1_0181512
  
  M0258S_L_v1_0181606
  
  M0258S_L_v1_0181612
  
  M0258S_L_v1_0181702
  
  M0258S_L_v1_0181706
  
  M0258S_L_v1_0181712
  
  M0258L_v2_10015886
  
  M0258S_v2_10017725
  
  M0258L_v2_10028994
  
  M0258L_v2_10034648
  
  M0258S_v2_10034093
  
  M0258L_v2_10054254
  
  M0258L_v2_10057547
  
  M0258S_v2_10055405
  
  M0258S_v2_10062085
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Deep Vent^® DNA Polymerase
  
  Magnesium Sulfate (MgSO₄) Solution
  
  ThermoPol^® Reaction Buffer Pack
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0258Datasheet-Lot0181206
  
  M0258Datasheet-Lot0181212
  
  M0258Datasheet-Lot0181406
  
  M0258Datasheet-Lot0181412
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Licenses
  Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
  Trademarks
  NEW ENGLAND BIOLABS®, VENT® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  DEEP VENT™ is a trademark of New England Biolabs, Inc.
  
  TRITON® is a registered trademark of Union Carbide Corporation

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Resources

Applications

Product Categories

You’ll be Thrilled to Pieces

Tools

Resources

Deep Vent^® DNA Polymerase

Product Source

Reagents Supplied

Features

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Companion Products

Materials Sold Separately

Product Citation Tool

Licenses

Trademarks

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Deep Vent® DNA Polymerase

Product Source

Reagents Supplied

Features

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Companion Products

Materials Sold Separately

Product Citation Tool

Licenses

Trademarks

Other Products You May Be Interested In

OneTaq® DNA Polymerase

OneTaq® Hot Start DNA Polymerase

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

OneTaq® Quick-Load® DNA Polymerase

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Ineligible item added to cart

Deep Vent^® DNA Polymerase

OneTaq^® Quick-Load^® DNA Polymerase

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer