NEBNext Direct® BRCA1/BRCA2 Panel
Target with precision.
NEBNext Direct employs a unique hybridization-based enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic variants while maximizing sequencer efficiency.
- Generate full (100%) coverage of all protein coding regions in BRCA1 and BRCA2 genes
- Obtain highly uniform sequencing across exon targets- 100% of base pairs in panel have coverage greater than 20% of the mean target coverage
- Maximize efficiency with a 1-day workflow that combines highly specific enrichment with library preparation
- Produce high depths of target coverage across a wide range of DNA input amounts for germline and somatic variant calling
Challenges and Opportunities for Next Generation Sequencing Target Enrichment
NEBNext Direct® Target Enrichment
NEBNext Direct Workflow
NEB TV Ep. 15 – Applications of Restriction Enzymes
The NEBNext Direct BRCA1/BRCA2 Panel enriches the complete exon content of BRCA1 and BRCA2 genes for next-generation sequencing analysis. NEBNext Direct employs a unique hybridization-based enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic variants while maximizing sequencer efficiency.
NEBNext Direct® BRCA1/BRCA2 Resources:
- GRCh37hg19 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
- GRCh38hg38 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
- Protocol for NEBNext Direct BRCA1/BRCA2 Panel (E6627)
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Sample Purification Beads NEBNext Direct BRCA1/BRCA2 Baits -20 NEBNext Direct® Bead Prep Buffer 4 NEBNext Direct® Hybridization Buffer 4 NEBNext Direct® dA-Tailing Buffer 4 NEBNext Direct® Adaptor Ligation Buffer 4 NEBNext Direct® Cleaving Buffer 4 NEBNext Direct® Hybridization Wash (HW) 4 NEBNext Direct® Bead Wash 1 25 NEBNext Direct® Bead Wash 2 4 NEBNext Direct® 3´ Adaptor -20 NEBNext Direct® 5´ UMI Adaptor -20 NEBNext Direct® dA-Tailing Enzyme -20 NEBNext Direct® Ligase -20 NEBNext Direct® 5´ Blunting Enzyme Mix -20 NEBNext Direct® Cleaving Enzyme Mix -20 NEBNext Direct® Q5 Master Mix -20 NEBNext Direct® FFPE Phosphorylation Enzyme -20 NEBNext Direct® Streptavidin Beads 4 NEBNext Direct® Hybridization Additive -20 NEBNext Direct® 5´ Blunting Buffer 4 NEBNext Direct® 3´ Blunting Enzyme Mix -20
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