The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|CutSmart® Buffer||-20||10 X|
|Yeast Carbon Base Medium Powder||4|
|Acetamide solution (sterile) (10 ml)||4||100 X|
|K. lactis GG799 Competent Cells||-80||5 reactions|
|NEB® Yeast Transformation Reagent (5 ml)||4||1 X|
|pKLAC1-malE Control Plasmid||-20||1,000 µg/ml|
|pKLAC2 Vector||-20||1,000 µg/ml|
|Integration Primer 1||-20||10 X|
|Integration Primer 2||-20||10 X|
|Integration Primer 3||-20||10 X|
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