NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
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NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
Chemically competent E. coli cells suitable for subcloning efficiency transformations, such as plasmid transformation or routine subcloning (e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency of these cells is not sufficiently high for most other cloning applications. For that, we recommend NEB® 5-alpha Competent E. coli (High Efficiency) (NEB #C2987) which has 1,000-fold higher transformation efficiency.
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Transformation efficiency > 1 x 106 cfu/µg pUC19 DNA
- Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
- Reduced recombination of cloned DNA (recA1)
- Resistance to phage T1 (fhuA2)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene
- K12 Strain
- DH5α™ derivative
- Free of animal products
GenotypefhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
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