RNA Purification from Buccal Swabs
- Collect cells from swab into 1X Monarch DNA/RNA Protection Reagent.
- For every 300 μl of DNA/RNA Protection Reagent/Sample mixture, add 15 μl Monarch Proteinase K. Vortex briefly and incubate at room temperature for 30 minutes.
- Vorex sample briefly and spin for 2 min (16,000 x g) to pellet debris. Transfer supernatant to an RNase-free microfuge tube (not included).
- Add an equal volume of Monarch RNA Lysis Buffer (NEB #T2012) and vortex briefly. Proceed to Step 1 of Part 2: RNA Binding and Elution in the Product Manual.