HomeFAQsIs it necessary to add a “G” into the oligo following the T7 promoter sequence?
FAQ: Is it necessary to add a “G” into the oligo following the T7 promoter sequence?
At least one G, directly downstream of the “TATA” of the T7 promoter
sequence is required for efficient transcription. This G will be the
+1 of the transcript. If the 20 nucleotide target-specific sequence
selected has a G at the 5’ end of the 20 nucleotides then it is not
necessary to add an additional G as the G within the target-specific
sequence will serve as the +1.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.