Historically the function of RNA in the cell was to assist in the translation of genetic information from DNA into protein. The major species of RNA were; (A) messenger RNA, which converts the genetic information of DNA into RNA, (B) transfer RNA (tRNA) that are charged with specific amino acids and, (C) ribosomal RNA (rRNA), a major component of the ribosome. RNA has now been implicated in a diverse number of biological processes including catalysis and transcriptional regulation. Recently, technological advances and improvements in RNA analysis and detection have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions. Examples include, microRNA (miRNA), circular RNA, long non-coding RNA (lncRNA), small nucleolar RNA (snoRNA) and extracellular RNA (exRNA). In addition, RNA modifications have revealed added complexity to RNA. These biologically relevant modifications are an active area of exploration. These findings have helped usher in a renaissance of RNA-focused research in biology.
RNA Analysis includes these areas of focus:
Latest NEB Expressions
This edition of NEB expressions focuses on tools that can streamline your RNA-related workflows. Featured products include NEBNext Ultra II kits for RNA, the Monarch Total RNA Miniprep Kit, Luna reagents for RT-qPCR, the new LunaScript RT SuperMix Kit and HiScribe kits for high yield, high quality RNA synthesis. Learn more about functional considerations for RNA synthesis in our feature, and learn about a new protocol for Cappable Seq. Lastly, read about ways you can promote sustainable science in the lab.
RNA Synthesis Brochure
Learn more about NEB's products for RNA synthesis, which range from template generation to poly(A) tailing.
RNA Technical Guide
Find in depth information on tools designed to streamline your RNA workflows, including RNA extraction & purification, qPCR and RT-qPCR, RNA-seq, RNA synthesis and gRNA synthesis.
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Avoiding Ribonuclease Contamination
- Guidelines for Purification of RNA from Cultured Mammalian Cells
Other Tools & Resources
- Fuchs, R.T., Sun, Z., Zhuang, F., et al. 2015. Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049
Publications related to RNA Analysis
Recommended HiScribe RNA Synthesis Kits by Application
RNA Polymerase Selection Chart
RNA Ligase Selection Chart
cDNA Synthesis Selection Chart
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.
This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene.