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NEBNext® Sample Preparation for Next Generation Sequencing

Preparation of high quality libraries at high yield is a critical first step in next generation sequencing workflows. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA and RNA and target enrichment of DNA for next generation sequencing. NEBNext kits are carefully designed to improve yields and library diversity using a broad range of input amounts from a wide variety of sample types. This has been most recently expanded with the addition of a novel enzyme-based DNA fragmentation system in the NEBNext Ultra II FS DNA Library Prep Kit (NEB #E7805), which enables reliable high-yield high-quality library construction with as little as 100 pg of input DNA.

Another recent technology addition to the NEBNext product line is NEBNext Direct® for target enrichment. This unique hybridization-based technology enables high sensitivity variant calling with the speed and precision previously only feasible using multiplex PCR-based approaches. The NEBNext Direct Cancer HotSpot Panel (NEB #E7000) provides highly specific hybridization-based capture of 190 common cancer targets from 50 genes, while the NEBNext Direct BRCA1/BRCA2 Panel (NEB #E6627) generates 100% coverage of all protein coding regions in BRCA1 and BRCA2 genes.


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NEBNext® Sample Preparation for Next Generation Sequencing includes these areas of focus:

Illumina® Library Preparation
RNA for Illumina®
DNA for Illumina®
NEBNext® Ultra™ II DNA Library Prep
NEBNext® Ultra™ II RNA Library Prep
Ion Torrent™ DNA Library Preparation
DNA for Ion Torrent™
DNA Library Preparation
RNA Library Preparation
DNA Fragmentation
FFPE DNA Repair
NEBNext FFPE DNA Repair Mix
NEBNext® Automation

FAQs for NEBNext® Sample Preparation for Next Generation Sequencing

Protocols for NEBNext® Sample Preparation for Next Generation Sequencing

    Publications related to NEBNext® Sample Preparation for Next Generation Sequencing

  1. Luck, A.N., Anderson, K.G., McClung, C.M., VerBerkmoes, N.C., Foster, J.M., Michalski, M.L. and Slatko, B.E. 2015. Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont BMC Genomics. , PubMedID: 26559510, DOI:

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.